一、FRET 熒光共振能量轉移供體與受體（淬滅受體）
A.Tide Fluor™ 染料——標記寡核苷酸和肽
Tide Fluor™ 染料是一系列高水溶性熒光試劑，主要用于標記寡核苷酸和肽，用于分子成像和診斷應用。使用 Tide Fluor™ 染料標記的偶聯物表現出異常明亮的熒光和更好的光穩定性，優于大多數傳統和專有的染料標記偶聯物，包括熒光素、花青素和大多數 Alexa Fluor? 染料標記偶聯物。為獲得 最佳FRET效果，Tide Fluor™ 染料應與互補的非熒光Tide Quencher™ 染料一起使用。Tide Fluor™-Tide Quencher™ 對之間的有效 FRET 作用有助于檢測納米范圍內的活細胞內的分子相互作用。
| 供體 | 可替代的常規染料 | Ex (nm) | Em (nm) | 貨號 （NHS酯） |
淬滅受體 | 貨號 （NHS酯）2 |
| Tide Fluor™ 1 [TF1] | EDANS | 341 | 448 | AAT-2244 | Tide Quencher™ 1[TQ1] | AAT-2199 |
| Tide Fluor™ 2WS [TF2WS] | Alexa Fluor??488 | 491 | 516 | AAT-2349 | Tide Quencher™ 2WS[TQ2WS] | AAT-2058 |
| Tide Fluor™ 2 [TF2] | FAM, FITC, Alexa Fluor??488 | 503 | 525 | AAT-2248 | Tide Quencher™ 2[TQ2] | AAT-2210 |
| Tide Fluor™ 3WS [TF3WS] | Cy3?, Alexa Fluor??555 | 551 | 563 | AAT-2346 | Tide Quencher™ 3WS[TQ3WS] | AAT-2229 |
| Tide Fluor™ 3 [TF3] | Cy3?, Alexa Fluor??555 | 554 | 578 | AAT-2271 | Tide Quencher™ 3[TQ3] | AAT-2230 |
| Tide Fluor™ 4 [TF4] | ROX, Texas Red?, Alexa Fluor??594 | 578 | 602 | AAT-2289 | Tide Quencher™ 4WS[TQ4WS] | AAT-2067 |
| Tide Fluor™ 5WS [TF5WS] | Cy5?, Alexa Fluor??647 | 649 | 664 | AAT-2281 | Tide Quencher™ 5WS[TQ5WS] | AAT-2081 |
| Tide Fluor™ 6WS [TF6WS] | Cy5.5?, IRDye??700, Alexa Fluor??680 | 682 | 701 | AAT-2294 | Tide Quencher™ 6WS[TQ6WS] | AAT-2096 |
| Tide Fluor™ 7WS [TF7WS] | Cy7?, Alexa Fluor??750 | 756 | 780 | AAT-2333 | Tide Quencher™ 7WS[TQ7WS] | AAT-2111 |
B.通用的廣譜淬滅受體
| 淬滅受體 | 可淬滅染料 | 貨號 |
| EQ0 NHS ester | AMCA, AMCA-X, DEAC, DEAC-X, MCA, MCC, CF350, CF405S, CF405M, Pacific Blue, Cascade Blue?, Marina Blue?, Alexa Fluor? 350, Alexa Fluor? 405, DyLight? 350, DyLight? 405, ATTO 390, ATTO 425, ATTO 465, iFluor350, iFLuor405, BD Horizon V450, BUV395, eFluor?450 | ELS-ENZ-CHM208-0001 |
| EQ1 NHS ester | FAM, Cy2, TET, JOE, VIC, HEX, Cy3, NED, TAMRA, Cy3.5, ROX, Texas Red, Quasar? 570, CAL Fluor? Gold 540, CAL Fluor? Orange 560, CAL Fluor? Red 590, CAL Fluor? Red 610, Alexa Fluor? 430, Alexa Fluor? 488, Alexa Fluor? 532, Alexa Fluor? 546, Alexa Fluor? 555, Alexa Fluor? 568, Alexa Fluor? 594, DyLight? 488, DyLight? 550, DyLight? 594, ATTO 488, ATTO 520, ATTO 532, ATTO Rho6G, ATTO 550, ATTO 565, ATTO Rho11, ATTO 590, ATTO 594. | ELS-ENZ-CHM165-0001 |
| EQ2 NHS ester | TET, JOE, VIC, HEX, Cy3, NED, TAMRA, Cy3.5, ROX, Texas Red, Cy5, Quasar? 570, Pulsar? 650, CAL Fluor? Gold 540, CAL Fluor? Orange 560, CAL Fluor? Red 590, CAL Fluor? Red 610, CAL Fluor? Red 635, Alexa Fluor? 532, Alexa Fluor? 546, Alexa Fluor? 555, Alexa Fluor? 568, Alexa Fluor? 594, Alexa Fluor? 610, Alexa Fluor? 633, Alexa Fluor? 647, DyLight? 550, DyLight? 594, DyLight? 633, DyLight? 650, ATTO 520, ATTO 532, ATTO Rho6G, ATTO 550, ATTO 565, ATTO Rho11, ATTO 590, ATTO 594, ATTO 633, ATTO Rho14, and ATTO 647 | ELS-ENZ-CHM166-0001 |
二、TR-FRET染料：
時間分辨-熒光共振能量轉移（TR-FRET）利用稀土元素中鑭系元素的獨特性質。在TR-FRET中常用的鑭系元素是釤（Sm）、銪（Eu）、鋱（Tb）、鏑（Dy）。與傳統熒光基團相比，他們具有大的斯托克位移，和非常長的發射半衰期（從微妙到毫秒），這使它們在生物學熒光應用領域中日益重要。
| 供體 | 貨號 | 受體 |
| trFluor™ Eu NHS酯(氨基反應性) | aat-1433 | APC iFluor™647 Tide Fluor™5 (TF5) Alexa Fluor?647 |
| trFluor™ Eu 馬來酰亞胺酯(巰基反應性) | aat-1434 | |
| trFluor™ Eu 鏈霉親和素(生物素反應性) | aat-16925 |
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